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Evidence Guide: MSL973015 - Prepare culture media

Student: __________________________________________________

Signature: _________________________________________________

Tips for gathering evidence to demonstrate your skills

The important thing to remember when gathering evidence is that the more evidence the better - that is, the more evidence you gather to demonstrate your skills, the more confident an assessor can be that you have learned the skills not just at one point in time, but are continuing to apply and develop those skills (as opposed to just learning for the test!). Furthermore, one piece of evidence that you collect will not usualy demonstrate all the required criteria for a unit of competency, whereas multiple overlapping pieces of evidence will usually do the trick!

From the Wiki University

 

MSL973015 - Prepare culture media

What evidence can you provide to prove your understanding of each of the following citeria?

Prepare culture media

  1. Prepare mixture of media and solvent to ensure solution and even settling of heat soluble materials
  2. Label media to allow tracking in subsequent processes
  3. Use a vessel large enough to endure adequate mixing and heating of the media
  4. Check pH of media where pH is critical
  5. Dispense media into vessels for sterilisation, leaving room for expansion during heating and cooling
Prepare mixture of media and solvent to ensure solution and even settling of heat soluble materials

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Label media to allow tracking in subsequent processes

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Use a vessel large enough to endure adequate mixing and heating of the media

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Check pH of media where pH is critical

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Dispense media into vessels for sterilisation, leaving room for expansion during heating and cooling

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Sterilise media

  1. Load the steriliser in keeping with maximum permitted loads and appropriate positioning of materials
  2. Ensure a sterilisation indicator is correctly placed with the load to monitor sterilisation process
  3. Operate sterilisation cycle in accordance with manufacturer requirements to achieve sterilisation at the required settings
  4. Cool media to the temperature specified in the media formulation procedures
  5. Sterilise media and/or media additives using membrane filtration techniques
Load the steriliser in keeping with maximum permitted loads and appropriate positioning of materials

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Ensure a sterilisation indicator is correctly placed with the load to monitor sterilisation process

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Operate sterilisation cycle in accordance with manufacturer requirements to achieve sterilisation at the required settings

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Cool media to the temperature specified in the media formulation procedures

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Sterilise media and/or media additives using membrane filtration techniques

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Label media

  1. Date batch media to ensure correct batch rotation
  2. Label media to allow for selection, avoiding areas of the culture vessel required for examination of colony growth
  3. Maintain records of batches of media
Date batch media to ensure correct batch rotation

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Label media to allow for selection, avoiding areas of the culture vessel required for examination of colony growth

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Maintain records of batches of media

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Label media to allow tracking in subsequent processes

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Use a vessel large enough to endure adequate mixing and heating of the media

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Check pH of media where pH is critical

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Dispense media into vessels for sterilisation, leaving room for expansion during heating and cooling

  1. Load the steriliser in keeping with maximum permitted loads and appropriate positioning of materials
Load the steriliser in keeping with maximum permitted loads and appropriate positioning of materials

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Ensure a sterilisation indicator is correctly placed with the load to monitor sterilisation process

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Operate sterilisation cycle in accordance with manufacturer requirements to achieve sterilisation at the required settings

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Cool media to the temperature specified in the media formulation procedures

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Sterilise media and/or media additives using membrane filtration techniques

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Dispense or aliquote media

  1. Add labile constituents where necessary, under conditions that will not lead to their denaturation or contamination of media
Add labile constituents where necessary, under conditions that will not lead to their denaturation or contamination of media

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Ensure even mixing of additives and media before dispensing

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Aseptically dispense media to minimise occurrence of procedural contamination

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Label media to allow for selection, avoiding areas of the culture vessel required for examination of colony growth

  1. Date batch media to ensure correct batch rotation
Date batch media to ensure correct batch rotation

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Maintain records of batches of media

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Store and incubate media

  1. Incubate control plates as a sterility check
Incubate control plates as a sterility check

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Store media to maximise shelf life and minimise contamination

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Perform quality control checks

  1. Inspect media for any evidence of possible contamination or problems with structure or sterilisation
Inspect media for any evidence of possible contamination or problems with structure or sterilisation

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Check useability of selective media by growth of expected organism

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Check stored stocks at regular intervals for conformance to required standards

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Maintain work area and equipment to prevent cross-infection and contamination

  1. Use personal protective equipment (PPE) and safe work practices to ensure personal safety and that of other laboratory personnel
Use personal protective equipment (PPE) and safe work practices to ensure personal safety and that of other laboratory personnel

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Place disposable and reusable items into relevant receptacles

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Clean and disinfect work area and equipment after use

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Transport disposable and reusable contaminated materials to relevant areas for disinfection, sterilisation and cleaning or disposal

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Assessed

Teacher: ___________________________________ Date: _________

Signature: ________________________________________________

Comments:

 

 

 

 

 

 

 

 

Instructions to Assessors

Required Skills and Knowledge